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API5 Polyclonal antibody

API5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25689-1-AP

Synonyms

AAC 11, AAC11, Antiapoptosis clone 11 protein, API 5, API5, apoptosis inhibitor 5, FIF, Protein XAGL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using API5 Polyclonal antibody (25689-1-AP)

    WB analysis using 25689-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using API5 Polyclonal antibody (25689-1-AP)

    WB analysis of HepG2 using 25689-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using API5 Polyclonal antibody (25689-1-AP)

    WB analysis of MCF-7 using 25689-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using API5 Polyclonal antibody (25689-1-AP)

    WB analysis of NIH/3T3 using 25689-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using API5 Polyclonal antibody (25689-1-AP)

    IHC staining of human lung using 25689-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using API5 Polyclonal antibody (25689-1-AP)

    IHC staining of human lung using 25689-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using API5 Polyclonal antibody (25689-1-AP)

    IF Staining of HepG2 using 25689-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using API5 antibody (25689-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using API5 Polyclonal antibody (25689-1-AP)

    IF Staining of HepG2 using 25689-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25689-1-AP (API5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25689-1-AP targets API5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen API5 fusion protein Ag22524
Full Name apoptosis inhibitor 5
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC017709
Gene Symbol API5
Gene ID (NCBI) 8539
RRIDAB_2880196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human gene API5 encodes the protein Apoptosis inhibitor 5. This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.

Protocols

Product Specific Protocols
WB protocol for API5 antibody 25689-1-APDownload protocol
IHC protocol for API5 antibody 25689-1-APDownload protocol
IF protocol for API5 antibody 25689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Sci Rep

microRNA regulatory circuits in a mouse model of inherited retinal degeneration.

Authors - Arpad Palfi
View Article
WB

Gene

Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.

Authors - Shuang Zhang
View Article
humanWB

Oncol Lett

Paeonol-mediated apoptosis of hepatocellular carcinoma cells by NF-κB pathway.

Authors - Qiang Li
View Article