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APOA5 Polyclonal antibody

APOA5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, pig, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18019-1-AP

Synonyms

Apo AV, APOA V, APOA5, APOAV, apolipoprotein A V, Apolipoprotein A5, RAP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOA5 Polyclonal antibody (18019-1-AP)

    WB analysis using 18019-1-AP

    HepG2 cell lysates were subjected to SDS PAGE followed by western blot with 18019-1-AP (APOA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using APOA5 Polyclonal antibody (18019-1-AP)

    WB analysis of pig liver using 18019-1-AP

    pig liver tissue were subjected to SDS PAGE followed by western blot with 18019-1-AP (APOA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 18019-1-AP is APOA5 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, pig, human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOA5 fusion protein Ag12568
Full Name apolipoprotein A-V
Calculated Molecular Weight 366 aa, 41 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC101787
Gene Symbol APOA5
Gene ID (NCBI) 116519
RRIDAB_2878483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APOA5 antibody 18019-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Transl Med

Characterization of the proteome of stable and unstable carotid atherosclerotic plaques using data-independent acquisition mass spectrometry

Authors - Zhichao Lai
View Article