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APOF Polyclonal antibody

APOF Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16608-1-AP

Synonyms

Apo F, APOF, apolipoprotein F, LTIP

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOF Polyclonal antibody (16608-1-AP)

    WB analysis of human plasma using 16608-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using APOF Polyclonal antibody (16608-1-AP)

    WB analysis of HepG2 using 16608-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOF Polyclonal antibody (16608-1-AP)

    IHC staining of human liver using 16608-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16608-1-AP (APOF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using APOF Polyclonal antibody (16608-1-AP)

    IHC staining of human liver using 16608-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16608-1-AP (APOF antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman plasma tissue, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16608-1-AP targets APOF in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOF fusion protein Ag9824
Full Name apolipoprotein F
Calculated Molecular Weight 326 aa, 35 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC026257
Gene Symbol APOF
Gene ID (NCBI) 319
RRIDAB_2058253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APOF antibody 16608-1-APDownload protocol
IHC protocol for APOF antibody 16608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma.

Authors - Zijian Da
View Article