• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ARFGAP3 Polyclonal antibody

ARFGAP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15293-1-AP

Synonyms

ARF GAP 3, ARFGAP1, ARFGAP3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of HepG2 using 15293-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of mouse brain using 15293-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of Jurkat using 15293-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IP experiment of HepG2 using 15293-1-AP

    IP result of anti-ARFGAP3 (IP:15293-1-AP, 4ug; Detection:15293-1-AP 1:2000) with HepG2 cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP

    Immunofluorescent analysis of A431 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of HepG2 using 15293-1-AP

    Immunofluorescent analysis of HepG2 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15293-1-AP targets ARFGAP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARFGAP3 fusion protein Ag7536
Full Name ADP-ribosylation factor GTPase activating protein 3
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-66 kDa
GenBank Accession NumberBC005122
Gene Symbol ARFGAP3
Gene ID (NCBI) 26286
RRIDAB_10804035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARFGAP3, also named as ADP-ribosylation factor GTPase-activating protein 3, is a 516 amino acid protein, which is Widely expressed with Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate). ARFGAP3 is a GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. The calculated molecular weight of ARFGAP3 is 57 kDa, but modified ARFGAP3 is about 57-66 kDa.

Protocols

Product Specific Protocols
WB protocol for ARFGAP3 antibody 15293-1-APDownload protocol
IHC protocol for ARFGAP3 antibody 15293-1-APDownload protocol
IF protocol for ARFGAP3 antibody 15293-1-APDownload protocol
IP protocol for ARFGAP3 antibody 15293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Biol

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Authors - Xihua Yue
  • KD Validated
View Article
humanWB

Commun Biol

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Authors - Xihua Yue
View Article
mouseIF

Cell Signal

ArfGAP3 regulates vesicle transport and glucose uptake in myoblasts

Authors - Suting Li
View Article