Arfaptin-1 Monoclonal antibody

Arfaptin-1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10B3

Cat no : 67461-1-Ig

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, RAW 264.7 cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue, HeLa cells, A549 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67461-1-Ig targets Arfaptin-1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Arfaptin-1 fusion protein Ag12097
Full Name ADP-ribosylation factor interacting protein 1
Calculated Molecular Weight 373 aa, 42 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC103759
Gene Symbol ARFIP1
Gene ID (NCBI) 27236
RRIDAB_2882691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
IHC protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
IF protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
FC protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols