• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ARFIP2 Polyclonal antibody

ARFIP2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14548-1-AP

Synonyms

Arfaptin 2, ARFIP2, Partner of RAC1, POR1, Protein POR1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of human brain using 14548-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of mouse pancreas using 14548-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of human liver using 14548-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    WB analysis of human heart using 14548-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human pancreas using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARFIP2 Polyclonal antibody (14548-1-AP)

    IHC staining of human pancreas using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    IF Staining of HeLa using 14548-1-AP

    Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARFIP2 Polyclonal antibody (14548-1-AP)

    IF Staining of HepG2 using 14548-1-AP

    Immunofluorescent analysis of HepG2 cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, human liver tissue, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14548-1-AP targets ARFIP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARFIP2 fusion protein Ag6076
Full Name ADP-ribosylation factor interacting protein 2
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa, 45 kDa
GenBank Accession NumberBC000392
Gene Symbol ARFIP2
Gene ID (NCBI) 23647
RRIDAB_2242930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARFIP2 antibody 14548-1-APDownload protocol
IHC protocol for ARFIP2 antibody 14548-1-APDownload protocol
IF protocol for ARFIP2 antibody 14548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,WB,IF

J Hepatocell Carcinoma

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway

Authors - Kaida Huang
View Article