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GRAF Polyclonal antibody

GRAF Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17747-1-AP

Synonyms

ARHGAP26, GRAF, KIAA0621, Oligophrenin 1 like protein, OPHN1L, OPHN1L1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of HeLa using 17747-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of mouse thymus using 17747-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of human brain using 17747-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GRAF Polyclonal antibody (17747-1-AP)

    WB analysis of HepG2 using 17747-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GRAF Polyclonal antibody (17747-1-AP)

    IP experiment of HepG2 using 17747-1-AP

    IP result of anti-GRAF (IP:17747-1-AP, 4ug; Detection:17747-1-AP 1:1000) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human liver using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human liver using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human heart using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GRAF Polyclonal antibody (17747-1-AP)

    IHC staining of human heart using 17747-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse thymus tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17747-1-AP targets GRAF in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRAF fusion protein Ag12008
Full Name Rho GTPase activating protein 26
Calculated Molecular Weight 759 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC068555
Gene Symbol ARHGAP26
Gene ID (NCBI) 23092
RRIDAB_2059284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GRAF antibody 17747-1-APDownload protocol
IHC protocol for GRAF antibody 17747-1-APDownload protocol
IP protocol for GRAF antibody 17747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neurol

Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis

Authors - Sven Jarius
View Article
humanWB

Brain Res

Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis.

Authors - Shufang Zhou
View Article
humanWB,IHC,CoIP

Cell Death Dis

CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway

Authors - Guojie Xu
View Article