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  • Featured Product
  • KD/KO Validated

ARIH2 Polyclonal antibody

ARIH2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15006-1-AP

Synonyms

ARI 2, ARI2, ariadne homolog 2 (Drosophila), ARIH2, Protein ariadne 2 homolog, TRIAD1, Triad1 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARIH2 Polyclonal antibody (15006-1-AP)

    WB analysis using 15006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15006-1-AP (ARIH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ARIH2 Polyclonal antibody (15006-1-AP)

    IP experiment of HeLa using 15006-1-AP

    IP result of anti-ARIH2 (IP:15006-1-AP, 4ug; Detection:15006-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ARIH2 Polyclonal antibody (15006-1-AP)

    IF Staining of HEK-293 using 15006-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15006-1-AP (ARIH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells, mouse brain tissue, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15006-1-AP targets ARIH2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARIH2 fusion protein Ag6507
Full Name ariadne homolog 2 (Drosophila)
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC000422
Gene Symbol ARIH2
Gene ID (NCBI) 10425
RRIDAB_2290024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.

Protocols

Product Specific Protocols
WB protocol for ARIH2 antibody 15006-1-APDownload protocol
IF protocol for ARIH2 antibody 15006-1-APDownload protocol
IP protocol for ARIH2 antibody 15006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Nat Immunol

ARIH2 is essential for embryogenesis, and its hematopoietic deficiency causes lethal activation of the immune system.

Authors - Lin Amy E AE
View Article
mouseWB

Oncogene

The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia.

Authors - Hao Wang
View Article
humanWB,IHC,IP

Cell Death Dis

ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.

Authors - Shengjun Geng
  • KD Validated
View Article