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ASAP3 Polyclonal antibody

ASAP3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14099-1-AP

Synonyms

ACAP4, ASAP3, CENTB6, DDEFL1, UPLC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASAP3 Polyclonal antibody (14099-1-AP)

    WB analysis using 14099-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14099-1-AP (ASAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASAP3 Polyclonal antibody (14099-1-AP)

    IF Staining of HepG2 using 14099-1-AP

    Immunofluorescent analysis of HepG2 cells, using ASAP3 antibody 14099-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14099-1-AP targets ASAP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASAP3 fusion protein Ag5231
Full Name ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Calculated Molecular Weight 903 aa, 99 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC060786
Gene Symbol ASAP3
Gene ID (NCBI) 55616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling.

Protocols

Product Specific Protocols
WB protocol for ASAP3 antibody 14099-1-APDownload protocol
IHC protocol for ASAP3 antibody 14099-1-APDownload protocol
IF protocol for ASAP3 antibody 14099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Aging (Albany NY)

Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.

Authors - Jiaming Fan
View Article