ASBT Polyclonal antibody

ASBT Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25245-1-AP

Synonyms

ASBT, IBAT, ISBT, NTCP2, SLC10A2



Tested Applications

Positive WB detected inmouse small intestine tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 10 publications below

Product Information

The immunogen of 25245-1-AP is ASBT Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse, Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASBT fusion protein Ag18808
Full Name solute carrier family 10 (sodium/bile acid cotransporter family), member 2
Calculated Molecular Weight 348 aa, 38 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC130523
Gene Symbol SLC10A2
Gene ID (NCBI) 6555
RRIDAB_2879986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ASBT antibody 25245-1-APDownload protocol
IP protocol for ASBT antibody 25245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Gastroenterology

Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.

Authors - Qiong Pan
mouseWB

Pharmacol Res

Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.

Authors - Jinxin Liu
mouseWB

J Agric Food Chem

Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.

Authors - Jinxin Liu
mouseWB

Food Funct

Apple polyphenol extract modulates bile acid metabolism and gut microbiota by regulating the circadian rhythms in daytime-restricted high fat diet feeding C57BL/6 male mice.

Authors - Yuan Cui
mouseWB

Biochim Biophys Acta

Rhizoma Coptidis alkaloids alleviate hyperlipidemia in B6 mice by modulating gut microbiota and bile acid pathways.

Authors - Kai He
WB

Biochim Biophys Acta Biomembr

S-acylation status of bile acid transporter hASBT regulates its function, metabolic stability, membrane expression, and phosphorylation state.

Authors - Ebehiremen N Ayewoh

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (12-01-2020)

It does not work as expected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse liver, mouse small intestine, mouse kidney, HepG2
ASBT Antibody Western Blot validation (1:500 dilution) in Mouse liver, mouse small intestine, mouse kidney, HepG2 (Cat no:25245-1-AP)