ASCC2 Polyclonal antibody

ASCC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11529-1-AP

Synonyms

ASC 1 complex subunit p100, ASC1p100, ASCC2, Trip4 complex subunit p100



Tested Applications

Positive WB detected inHEK-293T cells, human colon tissue, human brain tissue, HeLa cells, mouse colon tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

11529-1-AP targets ASCC2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASCC2 fusion protein Ag2100
Full Name activating signal cointegrator 1 complex subunit 2
Calculated Molecular Weight 757 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC025368
Gene Symbol ASCC2
Gene ID (NCBI) 84164
RRIDAB_2059358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ASCC2 antibody 11529-1-APDownload protocol
IHC protocol for ASCC2 antibody 11529-1-APDownload protocol
IF protocol for ASCC2 antibody 11529-1-APDownload protocol
IP protocol for ASCC2 antibody 11529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex

Authors - Junqiao Jia
WB

Nat Commun

The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations.

Authors - Junqiao Jia
mouseWB

Chin Med

Du Huo Ji Sheng Tang inhibits Notch1 signaling and subsequent NLRP3 activation to alleviate cartilage degradation in KOA mice

Authors - Wen-Jin Chen