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  • Featured Product
  • KD/KO Validated

ASF1A-specific Polyclonal antibody

ASF1A-specific Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 22259-1-AP

Synonyms

ASF1A, ASF1A-specific, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASF1A-specific Polyclonal antibody (22259-1-AP)

    WB analysis using 22259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ASF1A-specific Polyclonal antibody (22259-1-AP)

    IF Staining of NIH/3T3 using 22259-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22259-1-AP (ASF1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22259-1-AP targets ASF1A-specific in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF1A-specific fusion protein Ag17764
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2879052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.

Protocols

Product Specific Protocols
WB protocol for ASF1A-specific antibody 22259-1-APDownload protocol
IF protocol for ASF1A-specific antibody 22259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.

Authors - Sumin Feng
  • KD Validated
View Article