CoraLite®594-conjugated ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B7C11

Cat no : CL594-66346

Synonyms

ARSA, ARSA I, ARSA1, Arsenical pump driving ATPase, Arsenite stimulated ATPase, ASNA1, ATPase ASNA1, hARSA I, hASNA I, TRC40



Tested Applications

Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66346 targets ASNA1 in IF, FC (Intra) applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNA1 fusion protein Ag7936
Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Calculated Molecular Weight 38.7 kDa
Observed Molecular Weight 37-41 kDa
GenBank Accession NumberBC002651
Gene Symbol ASNA1
Gene ID (NCBI) 439
RRIDAB_2883564
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Protocols

Product Specific Protocols
IF protocol for CL594 ASNA1 antibody CL594-66346Download protocol
FC protocol for CL594 ASNA1 antibody CL594-66346Download protocol
Standard Protocols
Click here to view our Standard Protocols