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  • Featured Product
  • KD/KO Validated

ASPA Polyclonal antibody

ASPA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13244-1-AP

Synonyms

ACY 2, ACY2, Aminoacylase 2, ASP, ASPA, Aspartoacylase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ASPA Polyclonal antibody (13244-1-AP)

    WB analysis of mouse brain using 13244-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13244-1-AP (ASPA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ASPA Polyclonal antibody (13244-1-AP)

    IHC staining of human gliomas using 13244-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13244-1-AP (ASPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ASPA Polyclonal antibody (13244-1-AP)

    IHC staining of human gliomas using 13244-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13244-1-AP (ASPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13244-1-AP targets ASPA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASPA fusion protein Ag4029
Full Name aspartoacylase (Canavan disease)
Calculated Molecular Weight 313 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC029128
Gene Symbol ASPA
Gene ID (NCBI) 443
RRIDAB_2274358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASPA(Aspartoacylase) also called aminoacylase-2, is an enzyme that hydrolyzes N-acetyl-L-aspartic acid (NAA) to aspartate and acetate.Human ASPA encodes a deduced 313-amino acid protein with a molecular mass of 36 KDa. It shares 92% sequence identity with the bovine protein and contains 1 potential N-glycosylation site and 5 phosphorylation sites.Defects in ASPA are the cause of Canavan disease (CAND).This protein can exsit as a homodimer(18293939).

Protocols

Product Specific Protocols
WB protocol for ASPA antibody 13244-1-APDownload protocol
IHC protocol for ASPA antibody 13244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IHC,IF

Mil Med Res

Aspartoacylase suppresses prostate cancer progression by blocking LYN activation

Authors - Hong Weng
View Article
human,mouseWB

Elife

Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.

Authors - Vasiliki-Ilya Gargareta
View Article
mouseWB

J Neurosci

Suppressing N-Acetyl-l-Aspartate Synthesis Prevents Loss of Neurons in a Murine Model of Canavan Leukodystrophy.

Authors - Jiho Sohn
  • KO Validated
View Article
ratWB

Front Pharmacol

Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress.

Authors - Kaiyun Yao
View Article