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  • KD/KO Validated

ATP13A1 Polyclonal antibody

ATP13A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16244-1-AP

Synonyms

ATP13A, ATP13A1, ATPase type 13A1, CGI 152, KIAA1825



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16244-1-AP targets ATP13A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP13A1 fusion protein Ag9174
Full Name ATPase type 13A1
Calculated Molecular Weight 976 aa, 109 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC009302
Gene Symbol ATP13A1
Gene ID (NCBI) 57130
RRIDAB_2290293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATP13A1 antibody 16244-1-APDownload protocol
IHC protocol for ATP13A1 antibody 16244-1-APDownload protocol
IF protocol for ATP13A1 antibody 16244-1-APDownload protocol
IP protocol for ATP13A1 antibody 16244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Authors - Michael J McKenna
  • KO Validated
humanWB

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar
humanWB

J Cell Sci

Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex.

Authors - Peristera Roboti
  • KD Validated
humanWB

J Biol Chem

The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation.

Authors - Corinna A Kulicke
humanWB

Cell Rep

CATP-8/P5A ATPase Regulates ER Processing of the DMA-1 Receptor for Dendritic Branching.

Authors - Zhigang Feng
humanWB

Cell Rep

P5A ATPase controls ER translocation of Wnt in neuronal migration.

Authors - Tingting Li