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ATP5D Monoclonal antibody

ATP5D Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1G12C7

Cat no : 66673-1-Ig

Synonyms

ATP5D, F ATPase delta subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5D Monoclonal antibody (66673-1-Ig)

    WB analysis using 66673-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66673-1-Ig (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP5D Monoclonal antibody (66673-1-Ig)

    IHC staining of human kidney using 66673-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP5D Monoclonal antibody (66673-1-Ig)

    IHC staining of human kidney using 66673-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66673-1-Ig targets ATP5D in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5D fusion protein Ag7016
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 15-17 kDa
GenBank Accession NumberBC002389
Gene Symbol ATP5D
Gene ID (NCBI) 513
RRIDAB_2882027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.

Protocols

Product Specific Protocols
WB protocol for ATP5D antibody 66673-1-IgDownload protocol
IHC protocol for ATP5D antibody 66673-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols