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  • KD/KO Validated

ATP6V1C2 Polyclonal antibody

ATP6V1C2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16274-1-AP

Synonyms

ATP6C2, ATP6V1C2, V ATPase subunit C 2, VMA5



Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue
Positive IHC detected inhuman testis tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16274-1-AP targets ATP6V1C2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1C2 fusion protein Ag9349
Full Name ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2
Calculated Molecular Weight 381 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC012142
Gene Symbol ATP6V1C2
Gene ID (NCBI) 245973
RRIDAB_2062522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATP6V1C2 antibody 16274-1-APDownload protocol
IHC protocol for ATP6V1C2 antibody 16274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Environ Health Perspect

Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation.

Authors - Lishi Xie
  • KD Validated
humanWB,IHC

Front Genet

High Expression of ATP6V1C2 Predicts Unfavorable Overall Survival in Patients With Colon Adenocarcinoma

Authors - Guanghua Li