ATX3,ATXN3 Monoclonal antibody

ATX3,ATXN3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F7E10

Cat no : 67057-1-Ig

Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, pig brain tissue, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67057-1-Ig targets ATX3,ATXN3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATX3,ATXN3 fusion protein Ag6627
Full Name ataxin 3
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberBC033711
Gene Symbol ATXN3
Gene ID (NCBI) 4287
RRIDAB_2882368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Protocols

Product Specific Protocols
WB protocol for ATX3,ATXN3 antibody 67057-1-IgDownload protocol
IHC protocol for ATX3,ATXN3 antibody 67057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Clin Invest

CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion

Authors - Shengnan Wang