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AUTS2 Polyclonal antibody

AUTS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25001-1-AP

Synonyms

AUTS2, FBRSL2, KIAA0442

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of C6 cells using AUTS2 Polyclonal antibody (25001-1-AP)

    WB analysis of C6 using 25001-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using AUTS2 Polyclonal antibody (25001-1-AP)

    WB analysis of NIH/3T3 using 25001-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inC6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25001-1-AP is AUTS2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AUTS2 fusion protein Ag21853
Full Name autism susceptibility candidate 2
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 70 kDa, 130-140 kDa
GenBank Accession NumberBC064693
Gene Symbol AUTS2
Gene ID (NCBI) 26053
RRIDAB_2879840
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AUTS2 also named as FBRSL2 or autism susceptibility candidate 2 is a 1259 amino acid protein, which belongs to the AUTS2 family. AUTS2 is highly expressed in brain, kidney and skeletal muscle. AUTS2 exists as four isoforms produced by alternative splicing events and is encoded by a gene located on human chromosome 7. The molecular weight of AUTS2 isoform is 65-70 kDa in the brain ( PMID:23332918). The functional role of AUTS2 is not well known, although some studies have identified a putative role in transcriptional regulation during neuronal development.

Protocols

Product Specific Protocols
WB protocol for AUTS2 antibody 25001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Neurol

Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.

Authors - Jing Huang
View Article
humanIF

Neuroscience

PMID: 30599272

Authors - Fangkun Liu
View Article
mouse

Mol Cell

Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities.

Authors - Andrea Scelfo
View Article
mouseWB

Exp Neurol

Auts2 regulated autism-like behavior, glucose metabolism and oxidative stress in mice

Authors - Min Liu
View Article