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Alpha 2-Antiplasmin Monoclonal antibody

Alpha 2-Antiplasmin Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3F6C3

Cat no : 67308-1-Ig

Synonyms

A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, API, PLI, Serpin F2, SERPINF2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of HepG2 using 67308-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of HuH-7 using 67308-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of human plasma using 67308-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of THP-1 using 67308-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of K-562 using 67308-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    IHC staining of human liver using 67308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    IHC staining of human liver using 67308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    IF Staining of HepG2 using 67308-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human plasma, THP-1 cells, K-562 cells, HuH-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67308-1-Ig targets Alpha 2-Antiplasmin in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha 2-Antiplasmin fusion protein Ag4095
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Calculated Molecular Weight 491 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031592
Gene Symbol SERPINF2
Gene ID (NCBI) 5345
RRIDAB_2882569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
IHC protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
IF protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols