CoraLite®594-conjugated Annexin A1 Monoclonal antibody

Annexin A1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E1B7

Cat no : CL594-66344

Synonyms

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35



Tested Applications

Positive IF detected inA549 cells
Positive FC detected inK-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66344 targets Annexin A1 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin A1 fusion protein Ag17273
Full Name annexin A1
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001275
Gene Symbol ANXA1
Gene ID (NCBI) 301
RRIDAB_2883563
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Protocols

Product Specific Protocols
IF protocol for CL594 Annexin A1 antibody CL594-66344Download protocol
FC protocol for CL594 Annexin A1 antibody CL594-66344Download protocol
Standard Protocols
Click here to view our Standard Protocols