B3GNT5 Polyclonal antibody

B3GNT5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20422-1-AP

Synonyms

B3GN T5, B3GNT5, Beta 1,3 Gn T5, beta3Gn T5, BGnT 5, Lactotriaosylceramide synthase, Lc(3)Cer synthase, Lc3 synthase



Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20422-1-AP is B3GNT5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B3GNT5 fusion protein Ag14246
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Calculated Molecular Weight 378 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC028058
Gene Symbol B3GNT5
Gene ID (NCBI) 84002
RRIDAB_10694280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for B3GNT5 antibody 20422-1-APDownload protocol
IHC protocol for B3GNT5 antibody 20422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cell Mol Med

MiR-30a-5p inhibits proliferation and metastasis of hydatidiform mole by regulating B3GNT5 through ERK/AKT pathways.

Authors - Zhenzhen Guo
humanWB

Cell Rep

Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins

Authors - Jicheng Yang