B4GALNT2 Polyclonal antibody

B4GALNT2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24024-1-AP

Synonyms

B4GALGT2, B4GALNT2, B4GALT, Cad, GALGT2, SD, Sda



Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24024-1-AP is B4GALNT2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALNT2 fusion protein Ag21166
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 2
Calculated Molecular Weight 566 aa, 63 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC113677
Gene Symbol B4GALNT2
Gene ID (NCBI) 124872
RRIDAB_2879405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B4GALNT2, also named as GALGT2, catalyzes the last step in the biosynthesis of the human Sd(a) antigen, which is found on more than 90% of Caucasian red blood cells. It also catalyzes the last step in the biosynthesis of the Cad antigen, which is related both serologically and biochemically to the Sd(a) antigen. B4GALNT2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. This antibody detects the 55-65 kDa B4GALNT2 protein.

Protocols

Product Specific Protocols
WB protocol for B4GALNT2 antibody 24024-1-APDownload protocol
IHC protocol for B4GALNT2 antibody 24024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols