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B4GALNT3 Polyclonal antibody

B4GALNT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24675-1-AP

Synonyms

B4GalNac T3, B4GALNT3, Beta4GalNAc T3, Beta4GalNAcT3, NGalNAc T2

Validation Data Gallery

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  • Western Blot (WB) analysis of Transfected HEK-293 cells using B4GALNT3 Polyclonal antibody (24675-1-AP)

    WB analysis of Transfected HEK-293 using 24675-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24675-1-AP (B4GALNT3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using B4GALNT3 Polyclonal antibody (24675-1-AP)

    IHC staining of mouse stomach using 24675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using B4GALNT3 Polyclonal antibody (24675-1-AP)

    IHC staining of mouse stomach using 24675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 10x lens)..

Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24675-1-AP targets B4GALNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALNT3 fusion protein Ag20342
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 3
Calculated Molecular Weight 998 aa, 115 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC156653
Gene Symbol B4GALNT3
Gene ID (NCBI) 283358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B4GALNT3 (β1,4-N-acetylgalactosaminyltransferase III) has in vitro activity to promote the synthesis of LacdiNAc, an important structure found on glycoproteins expressed by neurons. B4GALNT3 was highly expressed in either differentiated NB or mature ganglion cells (PMID: 21741930). B4GALNT3 is predominantly expressed in the stomach, colon, and testis (PMID: 32267274).

Protocols

Product Specific Protocols
WB protocol for B4GALNT3 antibody 24675-1-APDownload protocol
IHC protocol for B4GALNT3 antibody 24675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols