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MKS1 Monoclonal antibody

MKS1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A8B11

Cat no : 66518-1-Ig

Synonyms

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MKS1 Monoclonal antibody (66518-1-Ig)

    WB analysis using 66518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    WB analysis of pig brain using 66518-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of human liver cancer using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MKS1 Monoclonal antibody (66518-1-Ig)

    IHC staining of human liver cancer using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNCCIT cells, pig brain tissue, HEK-293 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66518-1-Ig targets MKS1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MKS1 fusion protein Ag9504
Full Name Meckel syndrome, type 1
Calculated Molecular Weight 559 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010061
Gene Symbol MKS1
Gene ID (NCBI) 54903
RRIDAB_2881881
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MKS1 antibody 66518-1-IgDownload protocol
IHC protocol for MKS1 antibody 66518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols