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p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16815-1-AP

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, p130Cas

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    WB analysis using 16815-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    WB analysis of A431 using 16815-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of RAW 264.7 cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IP experiment of RAW 264.7 using 16815-1-AP

    IP result of anti-p130Cas / BCAR1 (IP:16815-1-AP, 4ug; Detection:16815-1-AP 1:600) with RAW 264.7 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IF Staining of HeLa using 16815-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using p130Cas / BCAR1 antibody (16815-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    FC experiment of A431 using 16815-1-AP

    1X10^6 A431 cells were stained with 0.2 ug Anti-Human p130Cas / BCAR1 (16815-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, T-47D cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16815-1-AP targets p130Cas / BCAR1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p130Cas / BCAR1 fusion protein Ag10354
Full Name breast cancer anti-estrogen resistance 1
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2065460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IF protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IP protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
FC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols