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BCAS1 Polyclonal antibody

BCAS1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 25776-1-AP

Synonyms

AIBC1, BCAS1, NABC1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using BCAS1 Polyclonal antibody (25776-1-AP)

    WB analysis of NIH/3T3 using 25776-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25776-1-AP (BCAS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BCAS1 Polyclonal antibody (25776-1-AP)

    WB analysis of MCF-7 using 25776-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25776-1-AP (BCAS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BCAS1 Polyclonal antibody (25776-1-AP)

    IF Staining of MCF-7 using 25776-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 25776-1-AP (BCAS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25776-1-AP is BCAS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAS1 fusion protein Ag22760
Full Name breast carcinoma amplified sequence 1
Calculated Molecular Weight 584 aa, 62 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC126348
Gene Symbol BCAS1
Gene ID (NCBI) 8537
RRIDAB_2880235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BCAS1 antibody 25776-1-APDownload protocol
IF protocol for BCAS1 antibody 25776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols