BCAT1/ECA39 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 13640-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 61 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
human kidney tissue, COLO 320 cells, mouse heart tissue, mouse brain tissue, rat heart tissue

Positive IHC detected in:
human lymphoma tissue, human colon cancer tissue, human heart tissue, human pancreas tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
branched chain aminotransferase 1, cytosolic

Calculated molecular weight:
320aa,36 kDa

Observed molecular weight:
43-45 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
BCAT1

Synonyms
BCAT1, ECA39, MECA39, PNAS 121, PP18, Protein ECA39
Background

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.


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