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  • KD/KO Validated

BCRP,ABCG2 Polyclonal antibody

BCRP,ABCG2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27286-1-AP

Synonyms

ABC15, ABCG2, ABCP, BCRP,ABCG2, BMDP, CD338, CDw338, EST157481, MRX, MXR, MXR1



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells, SGC-7901 cells, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27286-1-AP targets BCRP,ABCG2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCRP,ABCG2 fusion protein Ag25911
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC021281
Gene Symbol ABCG2
Gene ID (NCBI) 9429
RRIDAB_2880830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

Protocols

Product Specific Protocols
WB protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
IHC protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Drug Resist Updat

Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer

Authors - Wei Zhang
human,mouseWB,IHC

Mol Cancer

Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.

Authors - Jianyou Gu
human,mouseWB,IHC

PLoS Biol

A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance

Authors - Jiaojiao Hao
human,mouseWB,IHC

Mol Ther

CRISPR-mediated MECOM depletion retards tumor growth by reducing cancer stem cell properties in lung squamous cell carcinoma.

Authors - Yuanyuan Ma
mouseWB

Phytomedicine

Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice

Authors - Xiao-Li Guo
humanWB

Apoptosis

Pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4) leads to the activation of RhoGTPases promoting the malignant phenotypes of thyroid cancer

Authors - Qingling Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (09-12-2022)

Used in Western Blot at 1:3000 Dilution and it detected a band at 75 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: U2OS Osteosarcoma cells
BCRP,ABCG2 Antibody Western Blot validation (1:3000 dilution) in U2OS Osteosarcoma cells (Cat no:27286-1-AP)