BCRP/ABCG2 Monoclonal antibody

BCRP/ABCG2 Monoclonal Antibody for IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1D5F8

Cat no : 60080-1-Ig

Synonyms



Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inNCCIT cell

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

60080-1-Ig targets BCRP/ABCG2 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCRP/ABCG2 fusion protein Ag4869
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70-80 kDa, 140-160 kDa
GenBank Accession NumberBC021281
Gene Symbol ABCG2
Gene ID (NCBI) 9429
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

Publications

SpeciesApplicationTitle
human,mouseWB

J Agric Food Chem

Diosmetin Maintains Barrier Integrity by Reducing the Expression of ABCG2 in Colonic Epithelial Cells

Authors - Jinfan Liu
WB

Fish Shellfish Immunol

An ATP-binding cassette transporter G2 (CgABCG2) regulates the haemocyte proliferation by modulating the G1/S phase transition of cell cycle in oyster Crassostrea gigas

Authors - Simiao Yu
mouseWB

J Nutr Biochem

ABCG2 plays a central role in the dysregulation of 25-hydrovitamin D in Crohn's disease

Authors - Jiaxue Peng