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BDH1 Polyclonal antibody

Name: BDH1 Polyclonal antibody
Brand: Proteintech
SKU: 15417-1-AP

BDH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15417-1-AP

Synonyms

BDH, BDH1, SDR9C1

Validation Data Gallery

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AllWBIPIHCIF

WB analysis using 15417-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 15417-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15417-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15417-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15417-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HT-29 using 15417-1-AP
HT-29 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15417-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15417-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 15417-1-AP
IP result of anti-BDH1 (IP:15417-1-AP, 4ug; Detection:15417-1-AP 1:300) with mouse liver tissue lysate 4000ug.
IHC staining of rat brain using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15417-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15417-1-AP (BDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 15417-1-AP

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Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, rat liver tissue, HT-29 cells, human liver tissue, rat brain tissue, COLO 320 cells, mouse brain tissue, mouse heart tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	rat brain tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 21 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

15417-1-AP targets BDH1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BDH1 fusion protein Ag7705
Full Name	3-hydroxybutyrate dehydrogenase, type 1
Calculated Molecular Weight	38 kDa
Observed Molecular Weight	31 kDa
GenBank Accession Number	BC005844
Gene Symbol	BDH1
Gene ID (NCBI)	622
RRID	AB_2274683
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Protocols

Product Specific Protocols
WB protocol for BDH1 antibody 15417-1-AP	Download protocol
IHC protocol for BDH1 antibody 15417-1-AP	Download protocol
IF protocol for BDH1 antibody 15417-1-AP	Download protocol
IP protocol for BDH1 antibody 15417-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis. Authors - Patrycja Puchalska View Article
human	WB	Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. Authors - De Huang View Article
mouse	WB	Sci Adv Ketogenic diet uncovers differential metabolic plasticity of brain cells Authors - Tim Düking View Article
human	WB	Proc Natl Acad Sci U S A PMID: 29311302 Authors - Marcus D Goncalves View Article
human,mouse	WB	EMBO Mol Med Ketone body oxidation increases cardiac endothelial cell proliferation. Authors - Eva-Maria Weis View Article
mouse	WB	J Cell Biol CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. Authors - Désirée Schatton View Article

BDH1 Polyclonal antibody

BDH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 15417-1-AP

WB analysis using 15417-1-AP

WB analysis of human liver using 15417-1-AP

WB analysis of rat liver using 15417-1-AP

WB analysis of COLO 320 using 15417-1-AP

WB analysis of HT-29 using 15417-1-AP

WB analysis of rat brain using 15417-1-AP

WB analysis of mouse liver using 15417-1-AP

IP experiment of mouse liver using 15417-1-AP

IHC staining of rat brain using 15417-1-AP

IHC staining of human liver cancer using 15417-1-AP

IHC staining of human liver cancer using 15417-1-AP

IF Staining of HepG2 using 15417-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Ketogenic diet uncovers differential metabolic plasticity of brain cells

PMID: 29311302

Ketone body oxidation increases cardiac endothelial cell proliferation.

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.