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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated BID Monoclonal antibody

BID Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A2E5

Cat no : CL594-60301

Synonyms

BID, FP497, p11 BID, p22 BID

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated BID Monoclonal antibody (CL594-60301)

    WB analysis using CL594-60301

    Jurkat cells were subjected to SDS PAGE followed by western blot with CL594-60301 (BID antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60301 targets BID in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BID fusion protein Ag21005
Full Name BH3 interacting domain death agonist
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
RRIDAB_2919915
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).

Protocols

Product Specific Protocols
WB protocol for CL594 BID antibody CL594-60301Download protocol
Standard Protocols
Click here to view our Standard Protocols