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BIN3 Polyclonal antibody

BIN3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20186-1-AP

Synonyms

BIN3, bridging integrator 3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of Jurkat using 20186-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of HeLa using 20186-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of mouse testis using 20186-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of HEK-293 using 20186-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of A375 using 20186-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, A375 cells, HEK-293 cells, HeLa cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20186-1-AP is BIN3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN3 fusion protein Ag14091
Full Name bridging integrator 3
Calculated Molecular Weight 253 aa, 30 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC001223
Gene Symbol BIN3
Gene ID (NCBI) 55909
RRIDAB_10638782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIN3, also named as MEPCE and BCDIN3, belongs to the methyltransferase superfamily. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.

Protocols

Product Specific Protocols
WB protocol for BIN3 antibody 20186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols