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BLNK Polyclonal antibody

BLNK Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10855-1-AP

Synonyms

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using BLNK Polyclonal antibody (10855-1-AP)

    WB analysis of Raji using 10855-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10855-1-AP (BLNK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using BLNK Polyclonal antibody (10855-1-AP)

    WB analysis of Ramos using 10855-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 10855-1-AP (BLNK Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BLNK Polyclonal antibody (10855-1-AP)

    IHC staining of human tonsillitis using 10855-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10855-1-AP (BLNK Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BLNK Polyclonal antibody (10855-1-AP)

    IHC staining of human tonsillitis using 10855-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10855-1-AP (BLNK Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BLNK Polyclonal antibody (10855-1-AP)

    IHC staining of human pancreas cancer using 10855-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10855-1-AP (BLNK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BLNK Polyclonal antibody (10855-1-AP)

    IHC staining of human pancreas cancer using 10855-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10855-1-AP (BLNK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using BLNK Polyclonal antibody (10855-1-AP)

    IF Staining of NIH/3T3 using 10855-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10855-1-AP (BLNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRaji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10855-1-AP targets BLNK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLNK fusion protein Ag1290
Full Name B-cell linker
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 68 kDa, 70 kDa
GenBank Accession NumberBC018906
Gene Symbol BLNK
Gene ID (NCBI) 29760
RRIDAB_2274779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BLNK antibody 10855-1-APDownload protocol
IHC protocol for BLNK antibody 10855-1-APDownload protocol
IF protocol for BLNK antibody 10855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Cytokine

Silencing BLNK protects against interleukin-1β-induced chondrocyte injury through the NF-κB signaling pathway.

Authors - Yi Cheng
View Article
mouseWB

Aging (Albany NY)

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Authors - Yuqi Xin
View Article