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BMP15 Polyclonal antibody

BMP15 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18982-1-AP

Synonyms

BMP 15, BMP15, BMP-15, bone morphogenetic protein 15, GDF 9B, GDF9B, ODG2, POF4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BMP15 Polyclonal antibody (18982-1-AP)

    WB analysis of HeLa using 18982-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18982-1-AP (BMP15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BMP15 Polyclonal antibody (18982-1-AP)

    IHC staining of human prostate cancer using 18982-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BMP15 Polyclonal antibody (18982-1-AP)

    IHC staining of human prostate cancer using 18982-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BMP15 Polyclonal antibody (18982-1-AP)

    IHC staining of human prostate cancer using 18982-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BMP15 Polyclonal antibody (18982-1-AP)

    IHC staining of human prostate cancer using 18982-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18982-1-AP (BMP15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18982-1-AP targets BMP15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name bone morphogenetic protein 15
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_005448
Gene Symbol BMP15
Gene ID (NCBI) 9210
RRIDAB_2878570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMP15, also named as GDF9B, is a member of the bone morphogenetic protein family which is part of the transforming growth factor beta superfamily. The transforming growth factor beta superfamily includes large families of growth and differentiation factors. BMP15 may be involved follicular development and oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Mutation of BMP15 will cause of ovarian dysgenesis type 2 (ODG2) and premature ovarian failure type 4 (POF4).

Protocols

Product Specific Protocols
WB protocol for BMP15 antibody 18982-1-APDownload protocol
IHC protocol for BMP15 antibody 18982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Abnormal expression levels of BMP15/Smad1 are associated with granulosa cell apoptosis in patients with polycystic ovary syndrome.

Authors - Xiangrong Cui
View Article
mouseIHC

Proc Natl Acad Sci U S A

A rare germline BMP15 missense mutation causes hereditary ovarian immature teratoma in human

Authors - Yakun Liu
View Article