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BORIS Polyclonal antibody

BORIS Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11074-2-AP

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using BORIS Polyclonal antibody (11074-2-AP)

    WB analysis of PC-3 using 11074-2-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using BORIS Polyclonal antibody (11074-2-AP)

    WB analysis of rat testis using 11074-2-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using BORIS Polyclonal antibody (11074-2-AP)

    WB analysis of mouse testis using 11074-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BORIS Polyclonal antibody (11074-2-AP)

    WB analysis of HeLa using 11074-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BORIS Polyclonal antibody (11074-2-AP)

    IHC staining of human ovary tumor using 11074-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11074-2-AP (BORIS antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using BORIS Polyclonal antibody (11074-2-AP)

    IF Staining of SH-SY5Y using 11074-2-AP

    Immunofluorescent analysis of SH-SY5Y7 cells, using CTCFL antibody 11074-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BORIS Polyclonal antibody (11074-2-AP)

    IF Staining of HeLa using 11074-2-AP

    Immunofluorescent analysis of HeLa cells using 11074-2-AP (BORIS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11074-2-AP targets BORIS in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BORIS fusion protein Ag1547
Full Name CCCTC-binding factor (zinc finger protein)-like
Calculated Molecular Weight 82 kDa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC130486
Gene Symbol CTCFL
Gene ID (NCBI) 140690
RRIDAB_614983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. Present polyclonal anti-CFCFL antibody(11074-2-AP) is produced by immunizing animals with part of C-terminal chain of CTCFL and detect a 75-kDa band and a 55-kDa band.

Protocols

Product Specific Protocols
WB protocol for BORIS antibody 11074-2-APDownload protocol
IHC protocol for BORIS antibody 11074-2-APDownload protocol
IF protocol for BORIS antibody 11074-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols