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BPHL Polyclonal antibody

BPHL Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17974-1-AP

Synonyms

BPH RP, BPHL, MCNAA, VACVASE, Valacyclovir hydrolase, Valacyclovirase

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using BPHL Polyclonal antibody (17974-1-AP)

    WB analysis of human kidney using 17974-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using BPHL Polyclonal antibody (17974-1-AP)

    WB analysis of human liver using 17974-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using BPHL Polyclonal antibody (17974-1-AP)

    WB analysis of mouse kidney using 17974-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using BPHL Polyclonal antibody (17974-1-AP)

    WB analysis of mouse kidney using 17974-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BPHL Polyclonal antibody (17974-1-AP)

    WB analysis of MCF-7 using 17974-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human kidney using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human kidney using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human placenta using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human placenta using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human lung using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human lung using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human ovary using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human ovary using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human heart using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using BPHL Polyclonal antibody (17974-1-AP)

    IHC staining of human heart using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inhuman kidney tissue, human liver tissue, MCF-7 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17974-1-AP targets BPHL in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BPHL fusion protein Ag12085
Full Name biphenyl hydrolase-like (serine hydrolase)
Calculated Molecular Weight 22 kDa, 32 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC037778
Gene Symbol BPHL
Gene ID (NCBI) 670
RRIDAB_2274994
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BPHL antibody 17974-1-APDownload protocol
IHC protocol for BPHL antibody 17974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols