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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BRD8 Monoclonal antibody

BRD8 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5B6D6

Cat no : CL488-60121

Synonyms

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated BRD8 Monoclonal anti (CL488-60121)

    IF Staining of HepG2 using CL488-60121

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-60121 (BRD8 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60121 targets BRD8 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BRD8 fusion protein Ag0790
Full Name bromodomain containing 8
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC008039
Gene Symbol BRD8
Gene ID (NCBI) 10902
RRIDAB_2883104
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BRD8 antibody CL488-60121Download protocol
Standard Protocols
Click here to view our Standard Protocols