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  • Featured Product
  • KD/KO Validated

BRMS1 Polyclonal antibody

BRMS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16096-1-AP

Synonyms

BRMS1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using BRMS1 Polyclonal antibody (16096-1-AP)

    WB analysis of MCF-7 using 16096-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of MCF-7 cells using BRMS1 Polyclonal antibody (16096-1-AP)

    WB analysis of MCF-7 using 16096-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BRMS1 Polyclonal antibody (16096-1-AP)

    WB analysis of HEK-293 using 16096-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human brain tissue using BRMS1 Polyclonal antibody (16096-1-AP)

    IHC staining of human brain using 16096-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using BRMS1 Polyclonal antibody (16096-1-AP)

    IHC staining of human brain using 16096-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BRMS1 Polyclonal antibody (16096-1-AP)

    IF Staining of MCF-7 using 16096-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 16096-1-AP (BRMS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16096-1-AP targets BRMS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRMS1 fusion protein Ag8939
Full Name breast cancer metastasis suppressor 1
Calculated Molecular Weight 246 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC009834
Gene Symbol BRMS1
Gene ID (NCBI) 25855
RRIDAB_2878217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as a metastasis suppressor gene in human breast cancer. BRMS1 has since been reported to suppress metastasis in a variety of cancer types such as melanoma, ovarian carcinoma, and non-small lung cancer.

Protocols

Product Specific Protocols
WB protocol for BRMS1 antibody 16096-1-APDownload protocol
IHC protocol for BRMS1 antibody 16096-1-APDownload protocol
IF protocol for BRMS1 antibody 16096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Oncol Rep

BRMS1 participates in regulating cell sensitivity to DNA interstrand crosslink damage by interacting with FANCI.

Authors - Jianming Dou
  • KD Validated
View Article