BRUNOL6 Polyclonal antibody

BRUNOL6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19078-1-AP

Synonyms

Bruno like protein 6, BRUNOL6, CELF 6, CELF6, RNA binding protein BRUNOL 6



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue, pig brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19078-1-AP targets BRUNOL6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRUNOL6 fusion protein Ag4970
Full Name bruno-like 6, RNA binding protein (Drosophila)
Calculated Molecular Weight 481 aa, 50 kDa
Observed Molecular Weight 50 kDa, 39 kDa
GenBank Accession NumberBC030835
Gene Symbol BRUNOL6
Gene ID (NCBI) 60677
RRIDAB_2243662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IHC protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IF protocol for BRUNOL6 antibody 19078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cell Death Dis

Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Authors - Bowen Liu