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BTBD10 Monoclonal antibody

BTBD10 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

4A1F8

Cat no : 60129-1-Ig

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using BTBD10 Monoclonal antibody (60129-1-Ig)

    WB analysis of PC-3 using 60129-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60129-1-Ig (BTBD10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60129-1-Ig targets BTBD10 in ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BTBD10 fusion protein Ag10667
Full Name BTB (POZ) domain containing 10
Calculated Molecular Weight 475 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC017072
Gene Symbol BTBD10
Gene ID (NCBI) 84280
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic Acid/Ammonium Sulfate Precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTBD10 is a novel Akt-interacting protein with a BTB/POZ domain,which equally binds to any Akt. BTBD10 appears to behave as a suppressor of cell death including neuronal cell death related to ALS and an enhancer of cell growth via its positive regulation of Akt phosphorylation. The protein is expressed ubiquitously in vivo, distributing in cells as a unique cytoplasmic filamentous around the nucleus.