Bcl2 Monoclonal antibody
Bcl2 Monoclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
mouse and More (2)
Applications
WB, IP, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1B5B9
Cat no : 66799-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, RAW 264.7 cells, mouse brain tissue, C2C12 cells, 4T1 cells |
| Positive IF detected in | NIH/3T3 cells |
| Positive FC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 17 publications below |
| IP | See 1 publications below |
Product Information
66799-1-Ig targets Bcl2 in WB, IP, IF, FC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Bcl2 fusion protein Ag24261 |
| Full Name | B-cell leukemia/lymphoma 2 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | NM-009741 |
| Gene Symbol | Bcl2 |
| Gene ID (NCBI) | 12043 |
| RRID | AB_2882142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Bcl2 antibody 66799-1-Ig | Download protocol |
| IF protocol for Bcl2 antibody 66799-1-Ig | Download protocol |
| FC protocol for Bcl2 antibody 66799-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Puerarin prevents sepsis-associated encephalopathy by regulating the AKT1 pathway in microglia | ||
Biomed Pharmacother Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice | ||
Redox Biol Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. | ||
Antioxidants (Basel) Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress | ||
Cell Prolif Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy. | ||
Int J Mol Sci Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses |
