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  • Featured Product
  • KD/KO Validated

SLIRP Polyclonal antibody

SLIRP Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26006-1-AP

Synonyms

C14orf156, DC50, PD04872, SLIRP, SRA stem-loop-interacting RNA-binding protein, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLIRP Polyclonal antibody (26006-1-AP)

    WB analysis using 26006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using SLIRP Polyclonal antibody (26006-1-AP)

    WB analysis of MDA-MB-453s using 26006-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SLIRP Polyclonal antibody (26006-1-AP)

    WB analysis of HeLa using 26006-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SLIRP Polyclonal antibody (26006-1-AP)

    WB analysis of PC-3 using 26006-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLIRP Polyclonal antibody (26006-1-AP)

    IHC staining of human kidney using 26006-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLIRP Polyclonal antibody (26006-1-AP)

    IHC staining of mouse testis using 26006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLIRP Polyclonal antibody (26006-1-AP)

    IHC staining of mouse testis using 26006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLIRP Polyclonal antibody (26006-1-AP)

    IHC staining of human kidney using 26006-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SLIRP Polyclonal antibody (26006-1-AP)

    IF Staining of PC-3 using 26006-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SLIRP Polyclonal antibody (26006-1-AP)

    IF Staining of PC-3 using 26006-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SLIRP Polyclonal antibody (26006-1-AP)

    IF Staining of PC-3 using 26006-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 26006-1-AP (SLIRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, MDA-MB-453s cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26006-1-AP targets SLIRP in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLIRP fusion protein Ag22945
Full Name chromosome 14 open reading frame 156
Calculated Molecular Weight 109 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC017895
Gene Symbol C14orf156
Gene ID (NCBI) 81892
RRIDAB_2880332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLIRP, also named as C14orf156, DC23, DC50 and PD04872. It is an RNA-binding protein on steroid receptor RNA activator (SRA), and its expression is ubiquitous in human normal tissues. SLIRP can affect mitochondrial mRNA transcription and energy conversion. In addition, the loss or weakening of SLIRP destroys part of the structure of mitochondria, affects mitochondria energy metabolism, and increases sperm or cell oxidative stress damage (PMID: 33150185). SLIRP has 2 isoforms with the molecular mass of 12 kDa.

Protocols

Product Specific Protocols
WB protocol for SLIRP antibody 26006-1-APDownload protocol
IHC protocol for SLIRP antibody 26006-1-APDownload protocol
IF protocol for SLIRP antibody 26006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,CoIP

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei
  • KD Validated
View Article
humanWB

Methods Mol Biol

Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA

Authors - Kah Ying Ng
View Article
humanWB

J Transl Med

A traditional gynecological medicine inhibits ovarian cancer progression and eliminates cancer stem cells via the LRPPRC-OXPHOS axis

Authors - Ruibin Jiang
View Article
humanWB

Int J Mol Sci

Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells

Authors - Mariia V Baleva
View Article