RMC1 Polyclonal antibody

RMC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20111-1-AP

Synonyms

C18orf8, HsT2591, Mic 1, MIC1, RMC1



Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20111-1-AP targets RMC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RMC1 fusion protein Ag13904
Full Name chromosome 18 open reading frame 8
Calculated Molecular Weight 657 aa, 75 kDa
Observed Molecular Weight 75-80 kDa, 66 kDa
GenBank Accession NumberBC002950
Gene Symbol C18orf8
Gene ID (NCBI) 29919
RRIDAB_10646439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RMC1 antibody 20111-1-APDownload protocol
IHC protocol for RMC1 antibody 20111-1-APDownload protocol
IP protocol for RMC1 antibody 20111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols