C1QA Monoclonal antibody
C1QA Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1B10E3
Cat no : 67063-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue, human blood tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
67063-1-Ig targets C1QA in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | C1QA fusion protein Ag28622 |
| Full Name | complement component 1, q subcomponent, A chain |
| Calculated Molecular Weight | 245 aa, 26 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC030153 |
| Gene Symbol | C1QA |
| Gene ID (NCBI) | 712 |
| RRID | AB_2882373 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C1QA antibody 67063-1-Ig | Download protocol |
| IHC protocol for C1QA antibody 67063-1-Ig | Download protocol |
| IF protocol for C1QA antibody 67063-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Onco Targets Ther Prognostic Implications of the Complement Protein C1Q and Its Correlation with Immune Infiltrates in Osteosarcoma. | ||
Cells Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing | ||
Front Pharmacol The proportion of C1q-high and ISG15-high monocytes in the skin of patients with Behçet disease |
