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  • Featured Product
  • KD/KO Validated

CTRP1 Polyclonal antibody

CTRP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12209-1-AP

Synonyms

C1QTNF1, CTRP1, GIP, ZSIG37

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using CTRP1 Polyclonal antibody (12209-1-AP)

    WB analysis of human testis using 12209-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CTRP1 Polyclonal antibody (12209-1-AP)

    WB analysis using 12209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTRP1 Polyclonal antibody (12209-1-AP)

    IHC staining of human prostate cancer using 12209-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTRP1 Polyclonal antibody (12209-1-AP)

    IHC staining of human prostate cancer using 12209-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTRP1 Polyclonal antibody (12209-1-AP)

    IHC staining of human prostate cancer using 12209-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12209-1-AP (CTRP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CTRP1 Polyclonal antibody (12209-1-AP)

    IHC staining of human placenta using 12209-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CTRP1 Polyclonal antibody (12209-1-AP)

    IHC staining of human placenta using 12209-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTRP1 Polyclonal antibody (12209-1-AP)

    IF Staining of HepG2 using 12209-1-AP

    Immunofluorescent analysis of HepG2 cells, using C1QTNF1 antibody 12209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman testis tissue, J774A.1 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12209-1-AP targets CTRP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTRP1 fusion protein Ag2854
Full Name C1q and tumor necrosis factor related protein 1
Calculated Molecular Weight 281 aa, 32 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC021553
Gene Symbol C1QTNF1
Gene ID (NCBI) 114897
RRIDAB_10644328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1q and tumor necrosis factor related protein 1(CTRP1), also known as C1QTNF1, is a member of the CTRP superfamily. CTRP1 is a secreted glycoprotein synthesized by a variety of tissues and cells, such as adipose tissue and macrophages. CTRP1 is a paralog of adiponectin. CTRP1 plays a critical role in regulating inflammation and lipid metabolism and acts as a targeted treatment for atherosclerosis, heart failure, hypertension, NAFLD, and diabetes mellitus (PMID: 36812667).

Protocols

Product Specific Protocols
WB protocol for CTRP1 antibody 12209-1-APDownload protocol
IHC protocol for CTRP1 antibody 12209-1-APDownload protocol
IF protocol for CTRP1 antibody 12209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Cell Dev Biol

CTRP1 Attenuates Cerebral Ischemia/Reperfusion Injury via the PERK Signaling Pathway.

Authors - Huizhi Fei
  • KD Validated
View Article
humanWB

Biomed Res Int

Identification of CTRP1 as a Prognostic Biomarker and Oncogene in Human Glioblastoma.

Authors - Liyan Chen
  • KD Validated
View Article
humanIHC

Front Mol Biosci

High C1QTNF1 expression mediated by potential ncRNAs is associated with poor prognosis and tumor immunity in kidney renal clear cell carcinoma

Authors - Jiechuan Qiu
View Article