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C2 Polyclonal antibody

C2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 27175-1-AP

Synonyms

C2, C3/C5 convertase, CO2, Complement C2, complement component 2

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C2 Polyclonal antibody (27175-1-AP)

    WB analysis of A431 using 27175-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C2 Polyclonal antibody (27175-1-AP)

    WB analysis of HepG2 using 27175-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using C2 Polyclonal antibody (27175-1-AP)

    WB analysis of Raji using 27175-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C2 Polyclonal antibody (27175-1-AP)

    IHC staining of human liver using 27175-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using C2 Polyclonal antibody (27175-1-AP)

    IHC staining of human liver using 27175-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, HepG2 cells, Raji cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27175-1-AP is C2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2 fusion protein Ag25690
Full Name complement component 2
Observed Molecular Weight 70 kDa, 80 kDa
GenBank Accession NumberBC043484
Gene Symbol C2
Gene ID (NCBI) 717
RRIDAB_2880787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C2 antibody 27175-1-APDownload protocol
IHC protocol for C2 antibody 27175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
View Article
IHC

Insect Biochem Mol Biol

Sequence analysis and function of mosquito aeCCC2 and Drosophila Ncc83 orthologs.

Authors - Phu C Duong
View Article