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C20orf195 Polyclonal antibody

C20orf195 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16040-1-AP

Synonyms

C20orf195

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using C20orf195 Polyclonal antibody (16040-1-AP)

    WB analysis of SMMC-7721 using 16040-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C20orf195 Polyclonal antibody (16040-1-AP)

    WB analysis of HepG2 using 16040-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C20orf195 Polyclonal antibody (16040-1-AP)

    IHC staining of human prostate cancer using 16040-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using C20orf195 Polyclonal antibody (16040-1-AP)

    IHC staining of human prostate cancer using 16040-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C20orf195 Polyclonal antibody (16040-1-AP)

    IF Staining of HepG2 using 16040-1-AP

    Immunofluorescent analysis of HepG2 cells using 16040-1-AP (C20orf195 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16040-1-AP targets C20orf195 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C20orf195 fusion protein Ag8914
Full Name chromosome 20 open reading frame 195
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC000912
Gene Symbol C20orf195
Gene ID (NCBI) 79025
RRIDAB_2878209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C20orf195 antibody 16040-1-APDownload protocol
IHC protocol for C20orf195 antibody 16040-1-APDownload protocol
IF protocol for C20orf195 antibody 16040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols