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C5 Monoclonal antibody

C5 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2G8C9

Cat no : 66634-1-Ig

Synonyms

C5, Complement C5, complement component 5, CPAMD4

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C5 Monoclonal antibody (66634-1-Ig)

    WB analysis of human plasma using 66634-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C5 Monoclonal antibody (66634-1-Ig)

    IF Staining of HepG2 using 66634-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using C5 Monoclonal antibody (66634-1-Ig)

    FC experiment of HepG2 using 66634-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human C5 (66634-1-Ig, Clone: 2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66634-1-Ig targets C5 in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C5 fusion protein Ag17882
Full Name complement component 5
Calculated Molecular Weight 1676 aa, 188 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
RRIDAB_2881993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C5 antibody 66634-1-IgDownload protocol
IF protocol for C5 antibody 66634-1-IgDownload protocol
FC protocol for C5 antibody 66634-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Authors - Sipeng Li
View Article
humanWB

Sci Rep

Natural antibodies and CRP drive anaphylatoxin production by urate crystals.

Authors - Anne Kathrin Wessig
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Neil (Verified Customer) (05-10-2019)

Unfortunately the C5 antibody did not perform as well as I had hoped. Either the gel was not prepared properly, or the antibody bound indiscriminately to the gel.

  • Applications: Western Blot,
  • Cell Tissue Type: Synovial Fluid