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C6orf130 Polyclonal antibody

C6orf130 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25249-1-AP

Synonyms

C6orf130, OARD1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of PC-3 cells using C6orf130 Polyclonal antibody (25249-1-AP)

    WB analysis of PC-3 using 25249-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25249-1-AP (C6orf130 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using C6orf130 Polyclonal antibody (25249-1-AP)

    IF Staining of PC-3 using 25249-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25249-1-AP (C6orf130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using C6orf130 Polyclonal antibody (25249-1-AP)

    IF Staining of PC-3 using 25249-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using C6orf130 antibody (25249-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25249-1-AP targets C6orf130 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C6orf130 fusion protein Ag19382
Full Name chromosome 6 open reading frame 130
Calculated Molecular Weight 152 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC011709
Gene Symbol C6orf130
Gene ID (NCBI) 221443
RRIDAB_2753118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C6orf130 remains largely unknown. Catalog#25249-AP is a rabbit polyclonal antibody raised against the full-length of human C6orf130. The MW of this protein is 17 kDa, and this antibody specially recognises the 17 kDa protein.

Protocols

Product Specific Protocols
WB protocol for C6orf130 antibody 25249-1-APDownload protocol
IF protocol for C6orf130 antibody 25249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

The interplay of TARG1 and PARG protects against genomic instability

Authors - Joséphine Groslambert
  • KO Validated
View Article